Seidel H J, Pfeiffer S, Kreja L
Int J Cell Cloning. 1983 Oct;1(5):324-42. doi: 10.1002/stem.5530010502.
T-cell leukemias have been induced in adult BDF1 mice by 12 or 15 weeks of exposure to butylnitrosourea (BNU) in the drinking water. This led to a depression of CFU-S numbers and reduced T- and B-cell responses to mitogens. These parameters were then studied during the BNU-free preleukemic latency period in individual mice. At the same time, leukemic cells were traced in the thymus, the spleen, and the bone marrow by transplantation. In mice without leukemia and mice with leukemic cells in only one organ, there was a general tendency to normal CFU-S numbers and T- and B-cell responses with time after BNU, although control levels were reached in only a few of the mice. The reaction of mixed lymphocyte cultures (MLC) remained low during the latency period. In the thymus an imbalance of the Con A, PHA, and MLC responses was observed. Out of 25 mice with induced leukemia, 8 had leukemic cells in the thymus only and 2 in the marrow only. In mice with leukemic cells in all 3 hemopoietic organs and an enlargement of the spleen, a shift of CFU-S from the marrow to the spleen was observed.
通过让成年BDF1小鼠饮用含丁基亚硝脲（BNU）的水12或15周，诱导出了T细胞白血病。这导致集落形成单位 - 脾（CFU - S）数量减少，以及T细胞和B细胞对丝裂原的反应降低。然后在个体小鼠无BNU的白血病前期潜伏期研究这些参数。同时，通过移植在胸腺、脾脏和骨髓中追踪白血病细胞。在无白血病的小鼠和仅一个器官中有白血病细胞的小鼠中，BNU处理后随着时间推移，CFU - S数量以及T细胞和B细胞反应总体上有恢复正常的趋势，不过只有少数小鼠达到了对照水平。在潜伏期，混合淋巴细胞培养（MLC）反应仍然较低。在胸腺中观察到刀豆蛋白A（Con A）、植物血凝素（PHA）和MLC反应失衡。在25只诱导出白血病的小鼠中，8只仅在胸腺中有白血病细胞，2只仅在骨髓中有白血病细胞。在所有3个造血器官中有白血病细胞且脾脏肿大的小鼠中，观察到CFU - S从骨髓转移到了脾脏。
