粗糙脉孢菌细胞中丙酮酸羧化酶的定位
Localization of pyruvate carboxylase in the cells of Neurospora crassa.
作者信息
Nishikawa K, Kuwana H
出版信息
Experientia. 1984 Apr 15;40(4):356-7. doi: 10.1007/BF01952549.
PMID:6232147
Abstract
The cell wall of Neurospora crassa was digested enzymatically and the cytosolic and the mitochondrial fractions were separated. The activity of pyruvate carboxylase (EC 6.4.1.1) was detected entirely in the cytosolic fraction. This indicates that the location of pyruvate carboxylase of N. crassa is in the cytosol, but is not in the mitochondria; this is different from the situation in animal tissues.
摘要
粗糙脉孢菌的细胞壁经酶解消化，然后分离出胞质部分和线粒体部分。丙酮酸羧化酶（EC 6.4.1.1）的活性完全在胞质部分检测到。这表明粗糙脉孢菌中丙酮酸羧化酶的定位在细胞质中，而非线粒体中；这与动物组织中的情况不同。
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