丙酮酸羧化酶在产有机酸曲霉菌株中的定位
Localization of pyruvate carboxylase in organic acid-producing Aspergillus strains.
作者信息
Bercovitz A, Peleg Y, Battat E, Rokem J S, Goldberg I
机构信息
Department of Applied Microbiology, Hebrew University, Jerusalem, Israel.
出版信息
Appl Environ Microbiol. 1990 Jun;56(6):1594-7. doi: 10.1128/aem.56.6.1594-1597.1990.
Abstract
The localization of pyruvate carboxylase (cytosolic or mitochondrial) was studied in nine different Aspergillus species (14 strains). In some species (A. aculeatus, A. flavus, A. foetidus, A. nidulans, A. ochraceus, and A. sojae), the pyruvate carboxylase activity could be detected only in the cytosolic fraction of the cells. Pyruvate carboxylase has been found only in the mitochondrial fraction of two strains of Aspergillus wentii. In Aspergillus oryzae and in five strains of Aspergillus niger, pyruvate carboxylase activity was detected both in the mitochondrial fraction and in the cytosol. There was no quantitative or qualitative correlation between the activities of pyruvate carboxylase in the mitochondrial and cytosolic fractions of the cells and the ability of the various Aspergillus strains to accumulate different organic acids.
摘要
在9种不同的曲霉属物种（14个菌株）中研究了丙酮酸羧化酶（胞质或线粒体）的定位。在一些物种（刺孢曲霉、黄曲霉、臭曲霉、构巢曲霉、赭曲霉和酱油曲霉）中，仅在细胞的胞质部分检测到丙酮酸羧化酶活性。仅在两株米曲霉的线粒体部分发现了丙酮酸羧化酶。在米曲霉和五株黑曲霉中，在线粒体部分和胞质溶胶中均检测到丙酮酸羧化酶活性。细胞线粒体和胞质部分中丙酮酸羧化酶的活性与各种曲霉菌株积累不同有机酸的能力之间没有定量或定性的相关性。
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