Khansari N, Petrini M, Ambrogi F, Goldschmidt-Clermont P, Fudenberg H H
Immunobiology. 1984 Jan;166(1):1-11. doi: 10.1016/s0171-2985(84)80138-2.
The role of autologous rosette forming cells (ARFC) in humoral immune responses was studied using an in vitro system. While depletion of ARFCs from PBL resulted in a significant increase of either total IgG or anti-TT IgG, addition of these cells to the system decreased the production of immunoglobulin to a level comparable to that of unfractionated PBL. The majority of the ARFCs reacted with anti-Leu2a and anti-Leu8. In contrast, the majority of non-ARFCs reacted with Leu3a and only 10% with Leu8 monoclonal antibodies. Stimulation of unfractionated PBL with concanavalin A (ConA) resulted in an increase of the ARFC population. ConA stimulation also increased the number of cells reactive with anti-Leu2 and/or anti-Leu8. The autorosette population had a higher purine nucleoside phosphorylase (PNP) content than the non-ARFC population. Although the ARFC suppressed synthesis of antibody by B cell in vitro when they were mixed with either autologous or allogeneic B cells, a marked proliferation of non-B cells was evident. We conclude that at least two different subpopulations of T cells are capable of forming rosettes with autologous red blood cells.
利用体外系统研究了自体玫瑰花结形成细胞（ARFC）在体液免疫反应中的作用。从外周血淋巴细胞（PBL）中去除ARFC会导致总IgG或抗破伤风类毒素IgG显著增加，而将这些细胞添加到系统中会使免疫球蛋白的产生降低到与未分离的PBL相当的水平。大多数ARFC与抗Leu2a和抗Leu8反应。相反，大多数非ARFC与Leu3a反应，只有10%与Leu8单克隆抗体反应。用刀豆球蛋白A（ConA）刺激未分离的PBL会导致ARFC群体增加。ConA刺激还增加了与抗Leu2和/或抗Leu8反应的细胞数量。自体玫瑰花结群体的嘌呤核苷磷酸化酶（PNP）含量高于非ARFC群体。尽管当ARFC与自体或同种异体B细胞混合时，它们在体外抑制B细胞抗体的合成，但非B细胞明显增殖。我们得出结论，至少有两种不同的T细胞亚群能够与自体红细胞形成玫瑰花结。
