Ichikawa Y, Daniels J C
Cell Immunol. 1983 Feb 15;76(1):105-12. doi: 10.1016/0008-8749(83)90352-0.
The reactivity of mitogen-induced autologous rosette-forming cells (ARFC) to interleukin 2 (IL-2; T-cell growth factor) was studied in the present report. Both ARFC-enriched T cells and ARFC-depleted T cells, which were separated from concanavalin A (Con A)-activated T cells, were reactive to this factor. The IL-2 activity was absorbed by both ARFC-enriched and ARFC-depleted T cells, although ARFC-enriched T cells could absorb more IL-2 activity. Furthermore, ARFC were further inducible by IL-2 from non-ARFC. These results suggest the expression of the receptors for IL-2 on both ARFC and non-ARFC following mitogen stimulation. They further support the possibility that mitogen-induced ARFC, rather than being recruited only from such a minor T-cell subset as the spontaneous ARFC, are more likely the result of most T cells being responsive to mitogenic stimulation and expressing the receptors for autologous erythrocytes by the effects of IL-2 and mitogen.
本报告研究了丝裂原诱导的自体花环形成细胞（ARFC）对白介素2（IL-2；T细胞生长因子）的反应性。从伴刀豆球蛋白A（Con A）激活的T细胞中分离得到的富含ARFC的T细胞和去除ARFC的T细胞均对该因子有反应。IL-2活性可被富含ARFC的T细胞和去除ARFC的T细胞吸收，尽管富含ARFC的T细胞能吸收更多的IL-2活性。此外，IL-2可使非ARFC进一步诱导产生ARFC。这些结果提示丝裂原刺激后ARFC和非ARFC上均有IL-2受体表达。它们进一步支持了这样一种可能性，即丝裂原诱导的ARFC并非仅从自发ARFC这样的少数T细胞亚群募集而来，更可能是大多数T细胞对丝裂原刺激产生反应，并在IL-2和丝裂原的作用下表达自体红细胞受体的结果。
