人牙根尖肉芽肿中细胞浸润的原位特征。2. 补体成分C3b和C3d受体的显示

In situ characterization of cell infiltrates in human dental periapical granulomas. 2. Demonstration of receptors for the complement components C3b and C3d.

作者信息

Skaug N, Johannessen A C, Matre R, Nilsen R

出版信息

J Oral Pathol. 1984 Apr;13(2):111-9. doi: 10.1111/j.1600-0714.1984.tb01407.x.

DOI:10.1111/j.1600-0714.1984.tb01407.x

Abstract

The inflammatory cell infiltrates in human dental periapical granulomas were studied through the demonstration of receptors (R) for fragments C3b and C3d of the complement component C3, C3bR and C3dR were detected in cryostat sections by the closed chamber hemadsorption technique. Sheep erythrocytes sensitized with rabbit IgM antibody and coated with human C3B or C3d were used as indicator cells. Of the 62 granulomas studied, 23 showed strong or moderate C3bR activity, and weak C3dR activity was detected in 5 out of 25 specimens. The C3bR activity was abolished by treatment with 0.25 mM periodic acid, indicating that carbohydrate is a part of the C3bR. Using an antiserum to C3R as first layer in indirect immunofluorescence experiments, the C3R was localized to cell membranes. The low C3dR activity indicates that a minor part of the mononuclear cells in the human periapical granulomas were B lymphocytes.

摘要

通过补体成分C3的C3b和C3d片段受体（R）的显示，对人类根尖周肉芽肿中的炎性细胞浸润进行了研究，采用封闭腔室血细胞吸附技术在低温切片中检测C3bR和C3dR。用兔IgM抗体致敏并包被人C3B或C3d的绵羊红细胞用作指示细胞。在所研究的62个肉芽肿中，23个显示出强或中度的C3bR活性，在25个标本中的5个检测到弱的C3dR活性。用0.25 mM高碘酸处理后，C3bR活性消失，表明碳水化合物是C3bR的一部分。在间接免疫荧光实验中，使用抗C3R抗血清作为第一层，将C3R定位到细胞膜上。低C3dR活性表明人类根尖周肉芽肿中的单核细胞有一小部分是B淋巴细胞。