Matre R, Vedeler C A
J Immunol Methods. 1987 Jan 26;96(1):139-44. doi: 10.1016/0022-1759(87)90378-4.
A closed chamber haemadsorption (HA) technique with tissue sections and an indirect haemagglutination (IHA) technique with monoclonal anti-C3b receptor (CR1) antibody were used to measure CR1 on human erythrocytes (E) (ECR1). In the HA technique the indicator cells were sheep E coated with rabbit IgM antibodies (A) and various amounts of human C3b (EAC). The ECR1 activity was classified according to the degree of haemadsorption of EAC. High ECR1 activity was found in 24% of healthy blood donors, intermediate activity in 62%, low activity in 12% and no activity in 2% of the blood donors. The results obtained using the IHA technique showed a good correlation with the results obtained using the HA technique. Both techniques are sensitive and the results are highly reproducible. The techniques are easy to perform and do not require expensive reagents or equipment. The HA and IHA techniques therefore offer an alternative to other techniques for measuring ECR1 activity.
采用带有组织切片的封闭腔室血细胞吸附（HA）技术和带有单克隆抗C3b受体（CR1）抗体的间接血凝（IHA）技术来测定人红细胞（E）上的CR1（ECR1）。在HA技术中，指示细胞是用兔IgM抗体（A）和不同量的人C3b包被的绵羊红细胞（EAC）。根据EAC的血细胞吸附程度对ECR1活性进行分类。在24%的健康献血者中发现高ECR1活性，62%为中等活性，12%为低活性，2%的献血者无活性。使用IHA技术获得的结果与使用HA技术获得的结果具有良好的相关性。两种技术都很灵敏，结果具有高度可重复性。这些技术易于操作，不需要昂贵的试剂或设备。因此，HA和IHA技术为测量ECR1活性的其他技术提供了一种替代方法。
