McKnight S L, Kingsbury R C, Spence A, Smith M
Cell. 1984 May;37(1):253-62. doi: 10.1016/0092-8674(84)90321-0.
The two distal transcription signals of the herpesvirus tk gene share a common hexanucleotide sequence and function in a mutually dependent manner. We examined their roles by introducing corresponding base mutations into each hexanucleotide. The effects of mutations in the hexanucleotide of the first distal signal match the pattern of effects of the corresponding mutations in the second distal signal. These concordant patterns suggest that the hexanucleotide repeats are functionally analogous. There is one difference between the two distal signals. Disruption of the second distal signal concomitantly inactivates the first distal signal; disruption of the first distal signal leads to only partial inactivation of the second distal signal. When the positions of the two distal signals are switched, the partial dependence of the second distal signal is alleviated. We suggest a model for positive transcriptional control analogous to activation of the cl maintenance promoter of bacteriophage lambda by repressor.
疱疹病毒tk基因的两个远端转录信号共享一个共同的六核苷酸序列，并以相互依赖的方式发挥作用。我们通过将相应的碱基突变引入每个六核苷酸来研究它们的作用。第一个远端信号的六核苷酸中的突变效应与第二个远端信号中相应突变的效应模式相匹配。这些一致的模式表明六核苷酸重复在功能上是类似的。两个远端信号之间存在一个差异。第二个远端信号的破坏会同时使第一个远端信号失活；第一个远端信号的破坏只会导致第二个远端信号部分失活。当两个远端信号的位置互换时，第二个远端信号的部分依赖性会减轻。我们提出了一个类似于噬菌体λ的cl维持启动子被阻遏物激活的正转录调控模型。
