A regimen for obtaining mature human oocytes from donors for research into human fertilization in vitro.

Patients awaiting laparoscopic sterilization were canvassed to allow oocytes to be aspirated from their ovaries at the time of operation for the purpose of testing the fertilizing capacity of spermatozoa from clinically infertile men. Because these patients were volunteers, the standard techniques for stimulation, ovulation prediction, operation, and oocyte recovery had to be modified for maximum safety and for convenience to both patient and staff. Superovulation was induced with 100 mg or 150 mg clomiphene citrate on days 2 to 6 or 5 to 9 of the menstrual cycle. Final maturation of oocytes was stimulated with 5000 IU of human chorionic gonadotropin administered between days 11 and 15 of the cycle, and the operation was performed 34 to 36 hours later on the routine gynecology operation lists. Of the 157 patients canvassed , 47% were willing to donate oocytes to the project. Despite the variability of the stimulation regimens, the simplified operative technique, and the fact that more than half the patients were on oral contraceptives in the previous cycle, one or more oocytes were retrieved in 75% of the patients. At the initial assessment, 83% of these were considered suitable for in vitro fertilization.