Chutná J, Sládecek M, Holán V, Hasek M
Folia Biol (Praha). 1984;30(2):109-22.
B10.A male mice were grafted with H-2-incompatible murine B10.A(2R) skin allografts and treated with antithymocyte serum on days 2, 4, and 7 after transplantation. Repeated injections of cell-free tissue extracts from livers or spleens of B10.A(2R) mice were given in the standard doses, starting on the day of transplantation or on day 14 or day 28 after transplantation. The standard doses were the equivalents of material extracted from 40 mg or 80 mg of wet weight of liver or spleen tissue. Almost all of the regimens used in which antigen injections were begun on day 14 or day 28 after transplantation were successful and led to a marked prolongation in skin allograft survival. In some experimental groups most of the grafts survived 100 days after grafting and 8--33% grafts showed long-term survival in individual groups. The mechanism of this tolerance is mediated by suppressor cells which were characterized by means of anti-Thy 1.2 antibodies as T lymphocytes. the in vitro experiments have shown that cytotoxic cell precursors may be present in long-term tolerant mice and that they may be reactive to the tolerated antigens after sensitization.
B10.A雄性小鼠被移植了H-2不相容的鼠B10.A(2R)皮肤同种异体移植物，并在移植后第2、4和7天用抗胸腺细胞血清进行处理。从移植当天或移植后第14天或第28天开始，以标准剂量重复注射来自B10.A(2R)小鼠肝脏或脾脏的无细胞组织提取物。标准剂量相当于从40毫克或80毫克湿重的肝脏或脾脏组织中提取的物质。几乎所有在移植后第14天或第28天开始注射抗原的方案都是成功的，并导致皮肤同种异体移植物存活时间显著延长。在一些实验组中，大多数移植物在移植后存活了100天，并且在各个组中有8%至33%的移植物显示出长期存活。这种耐受性的机制是由抑制细胞介导的，这些抑制细胞通过抗Thy 1.2抗体被鉴定为T淋巴细胞。体外实验表明，长期耐受的小鼠体内可能存在细胞毒性细胞前体，并且它们在致敏后可能对耐受抗原产生反应。
