Page W J, Huyer M
J Bacteriol. 1984 May;158(2):496-502. doi: 10.1128/jb.158.2.496-502.1984.
Azotobacter vinelandii solubilized iron from certain minerals using only dihydroxybenzoic acid, which appeared to be produced constitutively. Solubilization of iron from other minerals required dihydroxybenzoic acid and the siderophore N,N'-bis-(2,3- dihydroxybenzoyl )-L-lysine ( azotochelin ) or these chelators plus the yellow-green fluorescent siderophore azotobactin . In addition to this sequential production of siderophores, cells also demonstrated partial to hyperproduction relative to the iron-limited control. The iron sources which caused partial derepression of the siderophores caused derepression of all the high-molecular-weight iron-repressible outer membrane proteins except a 77,000-molecular-weight protein, which appeared to be coordinated with azotobactin production. Increased siderophore production correlated with increased production of outer membrane proteins with molecular weights of 93,000, 85,000, and 77,000, but an 81,000-molecular-weight iron-repressible protein appeared at a constant level despite the degree of derepression. When iron was readily available, it appeared to complex with a 60,000-molecular-weight protein believed to form a surface layer on the A. vinelandii cell.
维涅兰德固氮菌仅利用二羟基苯甲酸就能从某些矿物质中溶解铁，二羟基苯甲酸似乎是组成型产生的。从其他矿物质中溶解铁需要二羟基苯甲酸和铁载体N,N'-双-(2,3-二羟基苯甲酰基)-L-赖氨酸（固氮菌素），或者这些螯合剂加上黄绿色荧光铁载体固氮菌素。除了这种铁载体的顺序产生外，相对于铁限制对照，细胞还表现出部分到过量产生。导致铁载体部分去阻遏的铁源会导致除一种77,000分子量的蛋白质外的所有高分子量铁可阻遏外膜蛋白去阻遏，这种77,000分子量的蛋白质似乎与固氮菌素的产生相关。铁载体产量的增加与分子量为93,000、85,000和77,000的外膜蛋白产量的增加相关，但尽管去阻遏程度不同，一种81,000分子量的铁可阻遏蛋白的产量却保持恒定。当铁容易获得时，它似乎与一种60,000分子量的蛋白质结合，据信这种蛋白质在维涅兰德固氮菌细胞上形成一层表面层。
