Dauenhauer S A, Hull R A, Williams R P
J Bacteriol. 1984 Jun;158(3):1128-32. doi: 10.1128/jb.158.3.1128-1132.1984.
Prodigiosin, the bright red pigment produced by many strains of Serratia marcescens, is synthesized by a bifurcated pathway that terminates in the enzymatic condensation of the two final products, a monopyrrole and a bipyrrole . Sau3A fragments of S. marcescens ( Nima ) DNA were introduced into a strain of Escherichia coli K-12 by use of the cosmid vector pHC79 , and transformed clones were selected based on resistance to ampicillin. Among 879 transformants screened, 2 could be induced to synthesize prodigiosin when supplied with either one or both terminal products of the bifurcated pathway. Data are presented to support the idea that production of prodigiosin is not usually mediated by a plasmid.
灵菌红素是许多粘质沙雷氏菌菌株产生的亮红色色素，它通过一条分叉途径合成，该途径最终以两种终产物（一种单吡咯和一种联吡咯）的酶促缩合反应结束。利用粘粒载体pHC79将粘质沙雷氏菌（Nima）DNA的Sau3A片段导入大肠杆菌K-12菌株，并根据对氨苄青霉素的抗性筛选转化克隆。在筛选的879个转化体中，有2个在提供分叉途径的一种或两种终产物时可被诱导合成灵菌红素。本文提供的数据支持了灵菌红素的产生通常不由质粒介导的观点。
