Stridh S, Datema R
Virology. 1984 May;135(1):293-6. doi: 10.1016/0042-6822(84)90139-9.
Foscarnet has previously been shown to inhibit influenza RNA polymerase activity. In this report the mode of inhibition of foscarnet has been investigated by enzyme-kinetic procedures and product analysis. Foscarnet shows noncompetitive inhibition with respect to ATP, CTP, and UTP, and a mixed inhibition with respect to GTP. In the presence of foscarnet the initiation of the mRNA synthesis can still occur, but the elongation is inhibited. The block of mRNA formation by foscarnet seems to occur after the synthesis of the 12-nucleotide-long conserved sequence found at the 5 prime end of the viral message.
膦甲酸先前已被证明可抑制流感病毒RNA聚合酶的活性。在本报告中，通过酶动力学方法和产物分析对膦甲酸的抑制模式进行了研究。膦甲酸对ATP、CTP和UTP表现出非竞争性抑制，对GTP表现出混合抑制。在膦甲酸存在的情况下，mRNA合成仍可起始，但延伸过程受到抑制。膦甲酸对mRNA形成的阻断似乎发生在病毒信息5′端发现的12个核苷酸长的保守序列合成之后。
