Tatsuta M, Yamamoto R, Yamamura H, Iishi H, Noguchi S, Ichii M, Okuda S
J Natl Cancer Inst. 1984 Jul;73(1):59-67.
The phototoxicity of hematoporphyrin derivatives (HPD) and dye-laser radiation on adenocarcinoma cells of the human stomach was examined by light and electron microscopy. Adenocarcinoma cells were obtained from human stomach tissue by endoscopic biopsy. The cells were incubated for 5 minutes in the patient's own serum that contained 0.6 mg HPD/ml and then were exposed to dye-laser radiation at 630 nm at an irradiance of 15 mW/cm2. Electron microscopy showed that cytotoxicity was mediated by mitochondrial damage, dilatation of the rough endoplasmic reticulum, and alterations of the nuclear membrane. The degenerative changes were greater and more frequent in poorly differentiated adenocarcinoma cells than in well-differentiated ones. No marked temperature rise was detected during irradiation. Neither the dye alone nor light alone had any effect. A singlet oxygen-trapping agent, 1,3-diphenylisobenzofuran, prevented adenocarcinoma cell degeneration that otherwise would result from exposure to HPD and dye-laser radiation. Thus singlet oxygen may be the cytotoxic agent in this system.
通过光学显微镜和电子显微镜检查了血卟啉衍生物（HPD）的光毒性以及染料激光辐射对人胃腺癌细胞的影响。通过内镜活检从人胃组织中获取腺癌细胞。将细胞在含有0.6mg HPD/ml的患者自身血清中孵育5分钟，然后以15mW/cm²的辐照度暴露于630nm的染料激光辐射下。电子显微镜显示细胞毒性是由线粒体损伤、粗面内质网扩张和核膜改变介导的。与高分化腺癌细胞相比，低分化腺癌细胞的退行性变化更严重且更频繁。照射期间未检测到明显的温度升高。单独的染料或单独的光均无任何作用。单线态氧捕获剂1,3 - 二苯基异苯并呋喃可防止腺癌细胞因暴露于HPD和染料激光辐射而发生变性。因此，单线态氧可能是该系统中的细胞毒性剂。
