Hanatani M, Nishifuji K, Futai M, Tsuchiya T
J Biochem. 1984 May;95(5):1349-53. doi: 10.1093/oxfordjournals.jbchem.a134742.
Alkanoyl-N-methylglucamides, nonionic detergents, were utilized to solubilize membrane proteins of Escherichia coli and were used to reconstitute them into liposomes. First, critical micelle concentrations (CMC) of nonanoyl-N-methylglucamide and decanoyl-N-methylglucamide were determined to be 25 mM and 7 mM, respectively, by photometric assay. Then solubilization and reconstitution of the melibiose transport carrier were performed using these detergents at concentrations above the CMC. Melibiose counterflow activity was observed with the proteoliposomes reconstituted from the extracted proteins and phospholipids. The proton-translocating ATPase complex (F1-F0) was also solubilized with these detergents. These results indicate that nonanoyl- and decanoyl-N-methylglucamide are useful detergents for solubilization and reconstitution of membrane proteins.
链烷酰 - N - 甲基葡糖酰胺，即非离子型去污剂，被用于溶解大肠杆菌的膜蛋白，并将其重新组装到脂质体中。首先，通过光度测定法确定壬酰 - N - 甲基葡糖酰胺和癸酰 - N - 甲基葡糖酰胺的临界胶束浓度（CMC）分别为25 mM和7 mM。然后，使用浓度高于CMC的这些去污剂进行蜜二糖转运载体的溶解和重组。从提取的蛋白质和磷脂重组得到的蛋白脂质体表现出蜜二糖逆流活性。质子转运ATP酶复合物（F1 - F0）也能用这些去污剂溶解。这些结果表明，壬酰和癸酰 - N - 甲基葡糖酰胺是用于溶解和重组膜蛋白的有效去污剂。
