正辛基-β-D-硫代吡喃葡萄糖苷的特性，一种可用于膜生物化学的新型非离子洗涤剂。
Characteristics of n-octyl beta-D-thioglucopyranoside, a new non-ionic detergent useful for membrane biochemistry.
作者信息
Saito S, Tsuchiya T
出版信息
Biochem J. 1984 Sep 15;222(3):829-32. doi: 10.1042/bj2220829.
Abstract
n-Octyl beta-D-thioglucopyranoside, a new non-ionic detergent, was synthesized. Properties, and applicability to membrane proteins, of this detergent were investigated. The detergent was easily removed by dialysis. The solubilizing power of this detergent for Escherichia coli membrane proteins was similar to that of n-octyl beta-D-glucopyranoside, which has been widely used in membrane biochemistry. No inactivation of proteins was observed after the solubilization. n-Octyl beta-D-thioglucopyranoside was superior to n-octyl beta-D-glucopyranoside in that it was much more stable and could be synthesized at much lower cost.
摘要
合成了一种新型非离子去污剂正辛基-β-D-硫代吡喃葡萄糖苷。研究了该去污剂的性质及其对膜蛋白的适用性。该去污剂可通过透析轻松去除。这种去污剂对大肠杆菌膜蛋白的增溶能力与已在膜生物化学中广泛使用的正辛基-β-D-吡喃葡萄糖苷相似。增溶后未观察到蛋白质失活。正辛基-β-D-硫代吡喃葡萄糖苷比正辛基-β-D-吡喃葡萄糖苷更具优势，因为它更稳定且合成成本低得多。
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