Runge H M, Bücke W, Neumann H A
Onkologie. 1984 May;7 Suppl 2:66-73. doi: 10.1159/000215518.
Methods and evaluation of the human tumor stem cell assay (HTSCA) are described. Advantages and disadvantages of the test system are elaborated. The in vitro/in vivo correlation in the drug screening of human ovarian carcinomas shows that the prediction of sensitivity to a cytotoxic agent is only possible in 64%. Prediction of drug resistance, however, seems to be possible in 95%. The number of patients that profit from the HTSCA seems to be only less than 10%. Our investigations describe the influence of various hormones and antiestrogens on the colony formation of human ovarian carcinoma cells. Tamoxifen and his major metabolite 4-hydroxy-tamoxifen were the most active agents. Both compounds inhibit the colony survival (70% at pharmacological concentrations) of 60% of the screened ovarian carcinomas. A significant correlation to the quantitative level of estrogen or progesterone receptors could not be proved. Colony formation of ovarian carcinoma cells was compared in the HTSCA as described by Hamburger and Salmon and in a methylcellulose-monolayer system. Our results show that the colony formation corresponds to the results of the original HTSCA: Cloning ovarian carcinoma cells in the methylcellulose-monolayer, however, seems to be technically easier and faster.
本文描述了人类肿瘤干细胞检测法（HTSCA）的方法及评估。阐述了该检测系统的优缺点。人类卵巢癌药物筛选中的体外/体内相关性表明，对细胞毒性药物敏感性的预测仅有64%的可能性。然而，耐药性的预测似乎有95%的可能性。从HTSCA中获益的患者数量似乎仅不到10%。我们的研究描述了各种激素和抗雌激素对人类卵巢癌细胞集落形成的影响。他莫昔芬及其主要代谢物4-羟基他莫昔芬是最具活性的药物。这两种化合物可抑制60%的筛查卵巢癌的集落存活（在药理浓度下为70%）。未能证明与雌激素或孕激素受体的定量水平存在显著相关性。在HTSCA中，按照Hamburger和Salmon的方法，以及在甲基纤维素单层系统中，对卵巢癌细胞的集落形成进行了比较。我们的结果表明，集落形成与原始HTSCA的结果一致：然而，在甲基纤维素单层中克隆卵巢癌细胞在技术上似乎更容易、更快。
