Buckley P J, Dickson S A
Am J Clin Pathol. 1984 Aug;82(2):167-72. doi: 10.1093/ajcp/82.2.167.
Most of the monoclonal antibodies (MoAbs) to T-lymphocyte subsets are, with few exceptions thought to recognize antigens (Ags) unique to T-cells. The authors examined the distribution of cells reacting with MoAbs to the helper/inducer (OKT4, anti-Leu-3a,b) and suppressor/cytotoxic (OKT8, anti-Leu-2a) T-lymphocyte subsets and to "pan T" Ags (anti-Leu-1, anti-Leu-4) in sections from frozen human spleen. In addition to recognizing lymphocytes in the T-zone (periarteriolar lymphoid sheaths), MoAbs to the helper/inducer and suppressor/cytotoxic T-cell subsets reacted distinctly with the red pulp sinusoidal lining cells. MoAbs to "pan T" Ags also reacted with T-zone lymphocytes but did not react with sinusoidal lining cells. MoAbs to the helper/inducer and suppressor/cytotoxic subsets of T-lymphocytes do not appear to identify Ags unique to these cells.
除少数例外情况外，大多数针对T淋巴细胞亚群的单克隆抗体（MoAb）被认为能识别T细胞特有的抗原（Ag）。作者检测了与MoAb反应的细胞在冷冻人脾脏切片中的分布情况，这些MoAb针对辅助/诱导性（OKT4、抗Leu-3a、b）和抑制/细胞毒性（OKT8、抗Leu-2a）T淋巴细胞亚群以及“全T”抗原（抗Leu-1、抗Leu-4）。除了识别T区（动脉周围淋巴鞘）中的淋巴细胞外，针对辅助/诱导性和抑制/细胞毒性T细胞亚群的MoAb与红髓窦内衬细胞有明显反应。针对“全T”抗原的MoAb也与T区淋巴细胞反应，但不与窦内衬细胞反应。针对T淋巴细胞辅助/诱导性和抑制/细胞毒性亚群的MoAb似乎并未识别出这些细胞特有的抗原。
