Rando R R, Young N
Biochem Biophys Res Commun. 1984 Jul 31;122(2):818-23. doi: 10.1016/s0006-291x(84)80107-2.
Protein kinase C is synergistically activated by the presence of calcium, certain phospholipids and a diacylglycerol. The physiological activation of the enzyme appears to be determined by the availability of the diacylglycerol which is itself a product of (poly) phosphoinositol turnover. It is shown here that the diacylglycerol activation effect is stereospecific, with only the 1,2-sn-diglycerides being active. This demonstrates for the first time a stereospecific effector role for a membrane-bound lipid. Furthermore, this work strengthens the link forged between the highly potent and specific tumor promoters (such as the phorbol esters) and the diglycerides as activators of protein kinase C.
蛋白激酶C在钙、某些磷脂和二酰基甘油存在的情况下会被协同激活。该酶的生理激活似乎取决于二酰基甘油的可获得性，而二酰基甘油本身是（多）磷酸肌醇周转的产物。本文表明，二酰基甘油的激活作用具有立体特异性，只有1,2-甘油二酯具有活性。这首次证明了膜结合脂质的立体特异性效应作用。此外，这项工作加强了高效和特异性肿瘤启动子（如佛波酯）与作为蛋白激酶C激活剂的甘油二酯之间的联系。
