Sentandreu R, Martinez-Ramon A, Ruiz-Herrera J
J Gen Microbiol. 1984 May;130(5):1193-9. doi: 10.1099/00221287-130-5-1193.
The localization of chitin synthase in the cells of Mucor rouxii was studied by a method which combined permeabilization of the cells with toluene/ethanol and incubation with the radioactive substrate UDP-[3H]GlcNAc followed by high resolution autoradiography. By this technique it was demonstrated that most of the chitin synthesized by these cells was located within the cytoplasm, and only a small amount of the enzyme product appeared at the cell surface. It was concluded that most of the chitin synthase of M. rouxii is located in the cytoplasm of the cells.
通过一种将细胞用甲苯/乙醇透化处理并与放射性底物UDP-[3H]GlcNAc孵育，随后进行高分辨率放射自显影的方法，研究了鲁氏毛霉细胞中几丁质合酶的定位。通过该技术表明，这些细胞合成的大部分几丁质位于细胞质内，只有少量酶产物出现在细胞表面。得出的结论是，鲁氏毛霉的大部分几丁质合酶位于细胞的细胞质中。
