Syrjänen S M, Syrjänen K J
Scand J Dent Res. 1984 Aug;92(4):275-81. doi: 10.1111/j.1600-0722.1984.tb00892.x.
Labial salivary glands of 51 patients with rheumatoid arthritis and those of 25 control patients were examined by the ANAE (acid alpha-naphthyl acetate esterase) technique to determine the percentages of B- and T-lymphocytes and mononuclear phagocytes (MPS cells). Using monoclonal antibodies (OKT3 for all T cells, OKT4 for helper/inducer T cells, OKT6 for thymocytes, and OKT8 for suppressor/cytotoxic T cells) T cell subsets were enumerated. B-lymphocytes predominated in both series of salivary glands, and the percentages of B and T cells were equal in both series. The absolute cell counts in the salivary glands of rheumatics were significantly higher (P less than 0.001) than in those of healthy controls. The number of OKT4+ cells was increased in rheumatics, leading to an elevated OKT4+/OKT8+ ratio when compared with that in controls (P less than 0.01). The results suggest that the basic phenomenon behind the B cell hyperactivity noticed in rheumatics might be due to increased activity of T helper cells rather than reduced number of T suppressor cells, which were shown to remain almost unaffected in the salivary glands of patients with rheumatoid arthritis.
采用酸性α-萘乙酸酯酶（ANAE）技术检测了51例类风湿性关节炎患者及25例对照患者的唇腺，以确定B淋巴细胞、T淋巴细胞和单核吞噬细胞（MPS细胞）的百分比。使用单克隆抗体（OKT3针对所有T细胞，OKT4针对辅助/诱导性T细胞，OKT6针对胸腺细胞，OKT8针对抑制/细胞毒性T细胞）对T细胞亚群进行计数。在两组唇腺中B淋巴细胞均占主导，且两组中B细胞和T细胞的百分比相等。类风湿患者唇腺中的绝对细胞计数显著高于健康对照组（P<0.001）。类风湿患者中OKT4+细胞数量增加，与对照组相比，导致OKT4+/OKT8+比值升高（P<0.01）。结果表明，类风湿患者中B细胞活性亢进背后的基本现象可能是由于辅助性T细胞活性增加，而非抑制性T细胞数量减少，后者在类风湿关节炎患者的唇腺中几乎未受影响。
