Ziegler M, Ziegler B, Hehmke B, Dietz H, Hildmann W, Kauert C
Biomed Biochim Acta. 1984;43(5):675-81.
Despite the existence of circumstantial evidence, a direct proof of an autoimmune basis for beta cell destruction in human type I diabetes has not yet been obtained. The present study was designed to test on Wistar rats whether a low-dose streptozotocin (SZ) treatment in combination with complete Freund's adjuvant (CFA) could be a useful approach to induce an autoimmune response to beta cells. Rats were weekly injected i.p. either with CFA or with SZ alone or with both CFA and SZ. Only the SZ-CFA-treated rats developed severe hyperglycemia. In these animals the pancreatic insulin content was nearly completely depleted. Only in SZ-CFA-treated rats cytotoxic autoantibodies to islet cells were found. The results show that in this new model of type I diabetes autoimmune reactions are involved in the destruction of beta cells.
尽管存在间接证据，但尚未获得人类I型糖尿病中β细胞破坏的自身免疫基础的直接证据。本研究旨在用Wistar大鼠测试低剂量链脲佐菌素（SZ）联合完全弗氏佐剂（CFA）治疗是否可能是诱导针对β细胞的自身免疫反应的有效方法。大鼠每周腹腔注射CFA或单独注射SZ或同时注射CFA和SZ。只有接受SZ-CFA治疗的大鼠出现严重高血糖。在这些动物中，胰腺胰岛素含量几乎完全耗尽。仅在接受SZ-CFA治疗的大鼠中发现了针对胰岛细胞的细胞毒性自身抗体。结果表明，在这种新的I型糖尿病模型中，自身免疫反应参与了β细胞的破坏。
