Ziegler M, Ziegler B, Hehmke B
Diabetologia. 1984 Jul;27 Suppl:163-5. doi: 10.1007/BF00275679.
The non-specific activation of the immune system by administration of complete Freund's adjuvant was examined in Wistar rats as a possible means of amplification of the specific immune response directed to pancreatic B cells caused by low dose non-diabetogenic multiple injections of streptozotocin. Rats were given intraperitoneally 0.5 ml of complete Freund's adjuvant to induce polyclonal lymphocyte activation and, 1 day later, the animals were given an intraperitoneal injection of 15 mg streptozotocin/kg body weight (group 1). This combined treatment was given twice at weekly intervals. In two further groups, rats were treated with complete Freund's adjuvant alone (group 2) or streptozotocin alone (group 3) with the same doses and at the same times. Only the rats in group 1 developed delayed but severe and persistent hyperglycaemia. In addition, significant complement-dependent cytotoxicity was detected in nine out of 15 rats (60%) in group 1 in islet cells, but not in spleen lymphocytes. The pancreatic insulin content of the rats in group 1 was depleted by up to 3.1 +/- 0.5%. With these experiments, a new animal model for insulin-dependent diabetes is described; complete Freund's adjuvant/streptozotocin diabetes. In many aspects, this model of diabetes parallels the development of insulin-dependent diabetes in man, including the humoral autoimmunity to islet cell antigens.
通过给予完全弗氏佐剂对Wistar大鼠免疫系统进行非特异性激活，以此作为一种可能的手段，来增强由低剂量非致糖尿病性多次注射链脲佐菌素引起的针对胰腺β细胞的特异性免疫反应。给大鼠腹腔注射0.5 ml完全弗氏佐剂以诱导多克隆淋巴细胞激活，1天后，给动物腹腔注射15 mg链脲佐菌素/千克体重（第1组）。这种联合治疗每周进行两次。另外两组大鼠，分别单独用完全弗氏佐剂（第2组）或单独用链脲佐菌素（第3组），剂量和时间相同。只有第1组的大鼠出现了延迟但严重且持续的高血糖症。此外，在第1组的15只大鼠中有9只（60%）的胰岛细胞中检测到显著的补体依赖性细胞毒性，但在脾淋巴细胞中未检测到。第1组大鼠的胰腺胰岛素含量最多减少了3.1±0.5%。通过这些实验，描述了一种新的胰岛素依赖型糖尿病动物模型；完全弗氏佐剂/链脲佐菌素糖尿病。在许多方面，这种糖尿病模型与人类胰岛素依赖型糖尿病的发展相似，包括对胰岛细胞抗原的体液自身免疫。
