Drexler H G, Gaedicke G
Leuk Res. 1983;7(5):611-9. doi: 10.1016/0145-2126(83)90132-7.
Enzymologic profiles of beta-hexosaminidase (N-acetyl-beta-D-glucosaminidase, E.C.3.2.1.30) were studied in cells from childhood acute leukemias and lymphomas. By analytical isoelectric focusing or disc electrophoresis the beta-hexosaminidase activity was separated into its components A, B, I and C. The isoenzyme patterns were correlated with immunologic cell surface marker characteristics found on the investigated leukemic cells. In all cases of T-ALL the beta-hexosaminidase forms A and B were observed, an enzyme pattern similar to that found in normal lymphocytes. Seven out of 11 cases with cALL, three of six cases with AML and one case of AUL displayed the intermediate component (Hex I). Marked heterogeneity within the immunologically classified subgroup cALL was reflected in different enzyme patterns of the cALL samples. These biochemical phenotypes may indicate the different maturation and differentiation status of cells expressing the same immunologic surface markers.
对儿童急性白血病和淋巴瘤细胞中的β-己糖胺酶（N-乙酰-β-D-氨基葡萄糖苷酶，E.C.3.2.1.30）的酶学特征进行了研究。通过分析等电聚焦或圆盘电泳，β-己糖胺酶活性被分离成其组分A、B、I和C。同工酶模式与在所研究的白血病细胞上发现的免疫细胞表面标志物特征相关。在所有T-ALL病例中，均观察到β-己糖胺酶形式A和B，其酶模式与正常淋巴细胞中的相似。11例cALL中的7例、6例AML中的3例和1例AUL显示出中间组分（Hex I）。免疫分类亚组cALL内的明显异质性反映在cALL样本的不同酶模式中。这些生化表型可能表明表达相同免疫表面标志物的细胞具有不同的成熟和分化状态。
