Koizumi H, Adachi K, Tsutsui M, Itami S, Katayama H, Halprin K M
J Invest Dermatol. 1984 Oct;83(4):261-4. doi: 10.1111/1523-1747.ep12340330.
We investigated calcium-activated, phospholipid-dependent protein kinase in pig epidermis. Pig epidermal homogenates were centrifuged at 30,000 g for 30 min, and the supernatant was applied on a DEAE-cellulose column for purification. The partially purified enzyme was stimulated by simultaneous addition of Ca2+ and phospholipid. Successive addition of small amounts of diolein further activated the enzyme activity. The calcium-activated phospholipid-dependent protein kinase preferentially phosphorylated serine residues and its endogenous substrate protein in the pig epidermis has a molecular weight of about 97,000.
我们研究了猪表皮中的钙激活磷脂依赖性蛋白激酶。将猪表皮匀浆在30,000g下离心30分钟，然后将上清液应用于DEAE - 纤维素柱进行纯化。同时添加Ca2+和磷脂可刺激部分纯化的酶。逐次添加少量二油精可进一步激活酶活性。钙激活磷脂依赖性蛋白激酶优先磷酸化丝氨酸残基，其在猪表皮中的内源性底物蛋白分子量约为97,000。
