Orlowski M, Sypherd P S
Biochemistry. 1978 Feb 21;17(4):569-75. doi: 10.1021/bi00597a002.
The present study was undertaken in order to elucidate the molecular mechanisms responsible for regulating changes in the specific rate of protein synthesis during the yeast-to-hyphae morphogenesis in the fungus, Mucor racemosus. The distribution of ribosomes between active polysomes and monosomes and inactive subunits was determined by means of pulse-labeling and density gradient fractionation techniques. The percentage of ribosomes active in protein synthesis was observed to decrease throughout the morphological transition. The rate of amino acid addition to nascent polypeptide chains was calculated and the transit time of messenger RNA translation was measured. The results showed a significant increase in the velocity of ribosome movement along the message which was continuously adjusted throughout hyphal development.
本研究旨在阐明在总状毛霉从酵母形态转变为菌丝形态过程中，负责调节蛋白质合成比速率变化的分子机制。通过脉冲标记和密度梯度分级分离技术，确定核糖体在活性多聚核糖体、单体核糖体和无活性亚基之间的分布。在整个形态转变过程中，观察到参与蛋白质合成的核糖体百分比下降。计算了氨基酸添加到新生多肽链上的速率，并测量了信使核糖核酸翻译的转运时间。结果表明，核糖体沿信使核糖核酸移动的速度显著增加，且在菌丝发育过程中不断调整。
