Hiatt W R, Inderlied C B, Sypherd P S
J Bacteriol. 1980 Mar;141(3):1350-9. doi: 10.1128/jb.141.3.1350-1359.1980.
The extent of differential gene expression during morphogenesis of Mucor racemosus was investigated by two-dimensional polyacrylamide gel electrophoresis of neutral and acidic polypeptides. Cellular proteins were labeled with [35S]methionine in cells growing in either the yeast or hyphal form, or in yeast cells undergoing the transition of hyphae. The results showed that of the 400 to 500 polypeptides resolved by electrophoresis, relatively few were specific to one or the other morphological form. The major change in the patterns of proteins synthesized during morphogenesis was a change in rates of synthesis of individual polypeptides. Experiments in which morphogenesis was affected under aerobic or anaerobic conditions showed that the majority of changes in the protein patterns were associated with morphogenesis and were not a specific response to O2.
通过对中性和酸性多肽进行二维聚丙烯酰胺凝胶电泳，研究了总状毛霉形态发生过程中差异基因表达的程度。在以酵母形式或菌丝形式生长的细胞中，或在经历向菌丝转变的酵母细胞中，用[35S]甲硫氨酸标记细胞蛋白。结果表明，在电泳分离出的400至500种多肽中，相对较少的多肽是某一种形态所特有的。形态发生过程中蛋白质合成模式的主要变化是个别多肽合成速率的改变。在有氧或无氧条件下影响形态发生的实验表明，蛋白质模式的大多数变化与形态发生相关，而不是对氧气的特异性反应。
