Johnson E A
Thromb Res. 1984 Sep 1;35(5):583-8. doi: 10.1016/0049-3848(84)90290-1.
A series of fractions of heparan sulphate has been prepared from the glycosaminoglycuronans of porcine intestinal mucosa, in order to relate their physicochemical properties with their biochemical and biological activities. They fall into three classes which may be distinguished by differences in molecular weight distribution and in extent of sulphation. For heterogeneous polymers such as heparan sulphates purification and fractionation must be regarded as interchangeable terms, and some preparative procedures appear to have rather specific effects on the properties of samples isolated by their use. Such considerations apply particularly in the undemarcated boundary region between heparan sulphates and heparin, a region which includes certain 'high activity' heparin fractions of current biological and clinical interest.
为了将硫酸乙酰肝素的物理化学性质与其生化和生物活性联系起来，已从猪肠黏膜的糖胺聚糖制备了一系列硫酸乙酰肝素级分。它们分为三类，可通过分子量分布和硫酸化程度的差异来区分。对于硫酸乙酰肝素等非均相聚合物，纯化和分级必须被视为可互换的术语，并且一些制备程序似乎对通过使用它们分离的样品的性质具有相当特定的影响。这些考虑尤其适用于硫酸乙酰肝素和肝素之间未划定的边界区域，该区域包括当前具有生物学和临床意义的某些“高活性”肝素级分。
