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毒力型结核分枝杆菌主要膜蛋白的特性分析

Characterization of the major membrane protein of virulent Mycobacterium tuberculosis.

作者信息

Lee B Y, Hefta S A, Brennan P J

机构信息

Department of Microbiology, Colorado State University, Fort Collins 80523.

出版信息

Infect Immun. 1992 May;60(5):2066-74. doi: 10.1128/iai.60.5.2066-2074.1992.

DOI:10.1128/iai.60.5.2066-2074.1992
PMID:1563797
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC257116/
Abstract

A protein with a molecular mass of 19 kDa was isolated and purified from enriched membrane fractions of the virulent Erdman strain of Mycobacterium tuberculosis. The protein is different from another 19-kDa protein, a lipoprotein, that was recently described (D. B. Young and T. R. Garbe, Res. Microbiol. 142:55-65, 1991). The sequencing strategy applied to this major membrane protein employed four different endoproteinases and resulted in sufficient overlapping peptide sequences for assignment of the entire protein sequence. Electron spray ionization mass spectrometry demonstrated a measured mass of 16,100, deviating from the predicted mass by only 2.86 atomic mass units. The sequence of this protein is unique. However, some similarities with other low-molecular-weight heat shock proteins were observed. Immunoblotting indicated that this protein is highly expressed in the virulent strains of M. tuberculosis. Its application to sera from patients with pulmonary tuberculosis showed promise as a serodiagnostic tool.

摘要

从结核分枝杆菌强毒株埃尔德曼株的富集膜组分中分离并纯化出一种分子量为19 kDa的蛋白质。该蛋白质与最近描述的另一种19 kDa蛋白质（一种脂蛋白）不同（D. B. 杨和T. R. 加贝，《微生物学研究》142:55 - 65，1991年）。应用于这种主要膜蛋白的测序策略使用了四种不同的内切蛋白酶，并产生了足够的重叠肽序列以确定整个蛋白质序列。电喷雾电离质谱法测得的质量为16,100，与预测质量仅相差2.86个原子质量单位。该蛋白质的序列是独特的。然而，观察到它与其他低分子量热休克蛋白有一些相似之处。免疫印迹表明该蛋白质在结核分枝杆菌的强毒株中高度表达。将其应用于肺结核患者的血清显示出作为血清诊断工具的前景。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c8e/257116/e0a80f0c38e5/iai00029-0359-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c8e/257116/1ffaa24b73f3/iai00029-0354-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c8e/257116/806f891bf24e/iai00029-0354-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c8e/257116/6eadd13b5aa6/iai00029-0355-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c8e/257116/9f252df0ed51/iai00029-0355-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c8e/257116/e0a80f0c38e5/iai00029-0359-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c8e/257116/1ffaa24b73f3/iai00029-0354-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c8e/257116/806f891bf24e/iai00029-0354-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c8e/257116/6eadd13b5aa6/iai00029-0355-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c8e/257116/9f252df0ed51/iai00029-0355-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c8e/257116/e0a80f0c38e5/iai00029-0359-a.jpg

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本文引用的文献

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Structural studies on the myo-inositol phospholipids of Mycobacterium tuberculosis (var. bovis, strain BCG).结核分枝杆菌（牛型，卡介苗菌株）的肌醇磷脂结构研究
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计算机模拟功能和结构表征揭示了菌株SCHU4的毒力蛋白。
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