Knop J, Stremmer R, Taborski U, Freitag W, de Maeyer-Guignard J, Macher E
J Immunol. 1984 Nov;133(5):2412-6.
The effects of electrophoretically pure murine interferon (Mu-IFN-alpha beta) on the T suppressor pathway and on the T effector cell of delayed hypersensitivity (TDH) were investigated in BALB/c mice, in a 2,4-dinitrofluorobenzene (DNFB) contact-sensitivity model. Various T cell subpopulations, suppressor T cells of the afferent (Ts-aff) and efferent (Ts-eff) types, an auxiliary Ts (Ts-aux), as well as TDH were induced, and their function was assessed in transfer experiments. The results were as follows. At a dose of 5 X 10(3) U, IFN was shown to inhibit the Ts-aff response, when given to the donor animal shortly after induction of the Ts-aff subpopulation or when injected into the recipient 2 hr after spleen cell transfer. Pretreatment in vitro with IFN of the splenic cells to be transferred also abolished the Ts-aff response. Similar amounts of IFN were able to inhibit the generation of Ts-eff in the donor animals, whereas 10-fold-higher amounts were needed in vivo or in vitro to block the functional expression of Ts-eff in the recipient animal. Intravenous injection of IFN into recipients of Ts-eff on day 0 and 1 after sensitization inhibited the expression of the Ts-eff transferred 1 day before ear challenge. This suggests that the Ts-aux response required for the TDH suppression by Ts-eff is blocked by IFN. Secretion of a suppressor factor by Ts in vitro was not blocked by IFN. Treatment of the donor of suppressor factor-secreting Ts with IFN, however, blocked the induction of this Ts. The TDH were not sensitive to IFN even at amounts approximately 100 times higher than those used for the Ts inhibition in vivo as well as in vitro. These results demonstrate that low amounts of IFN may selectively block the suppressor pathway, because induction of these regulatory T cell subsets appears to be particularly sensitive to IFN. The exact mechanism of the IFN-mediated inhibition of Ts is not yet clear. The data suggest an important regulatory function of IFN in delayed-type hypersensitivity (DTH) reactions.
在2,4-二硝基氟苯（DNFB）接触敏感性模型中，研究了电泳纯的小鼠干扰素（Mu-IFN-αβ）对BALB/c小鼠中T抑制途径和迟发型超敏反应（TDH）的T效应细胞的影响。诱导了各种T细胞亚群，传入型（Ts-aff）和传出型（Ts-eff）抑制性T细胞、辅助性Ts（Ts-aux）以及TDH，并在转移实验中评估了它们的功能。结果如下。在剂量为5×10³ U时，当在诱导Ts-aff亚群后不久给予供体动物IFN，或在脾细胞转移后2小时注入受体时，IFN可抑制Ts-aff反应。待转移的脾细胞在体外经IFN预处理也消除了Ts-aff反应。相似量的IFN能够抑制供体动物中Ts-eff的产生，而在体内或体外需要高出10倍的量来阻断受体动物中Ts-eff的功能表达。在致敏后第0天和第1天向Ts-eff受体静脉注射IFN，抑制了在耳部激发前1天转移的Ts-eff的表达。这表明Ts-eff抑制TDH所需的Ts-aux反应被IFN阻断。Ts在体外分泌抑制因子未被IFN阻断。然而，用IFN处理分泌抑制因子的Ts的供体，可阻断该Ts的诱导。TDH即使在比体内和体外抑制Ts所用剂量高约100倍的量时也对IFN不敏感。这些结果表明，低量的IFN可能选择性地阻断抑制途径，因为这些调节性T细胞亚群的诱导似乎对IFN特别敏感。IFN介导的Ts抑制的确切机制尚不清楚。数据表明IFN在迟发型超敏反应（DTH）中具有重要的调节功能。
