交叉弛豫在蛋白质质子核磁共振自旋晶格弛豫时间测量中的作用的实验证据。
Experimental evidence for the role of cross-relaxation in proton nuclear magnetic resonance spin lattice relaxation time measurements in proteins.
作者信息
Sykes B D, Hull W E, Snyder G H
出版信息
Biophys J. 1978 Feb;21(2):137-46. doi: 10.1016/S0006-3495(78)85514-3.
Abstract
Proton nuclear magnetic resonance (NMR) spin lattice relaxation time (T1) and spin-spin relaxation time (T2) measurements are presented for a number of proteins with molecular weights spanning the range of 6,500-150,000 daltons. These measurements provide experimental evidence for the role of cross-relaxation in 1H NMR T1 measurements in proteins. The relationship between these measurements and the theory recently presented by Kalk and Berendsen is discussed. The results indicate that cross-relaxation dominates the T1 measurements for the larger proteins, even at relatively low resonance frequencies such as 100 MHz.
摘要
本文给出了一系列分子量在6500至150000道尔顿范围内的蛋白质的质子核磁共振（NMR）自旋晶格弛豫时间（T1）和自旋-自旋弛豫时间（T2）测量结果。这些测量为交叉弛豫在蛋白质1H NMR T1测量中的作用提供了实验证据。讨论了这些测量结果与Kalk和Berendsen最近提出的理论之间的关系。结果表明，即使在相对较低的共振频率（如100 MHz）下，交叉弛豫在较大蛋白质的T1测量中也占主导地位。
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本文引用的文献
1
Effect of internal rotation on nuclear magnetic relaxation times for macromolecules.内旋转对大分子核磁共振弛豫时间的影响。
Biochemistry. 1972 Oct 10;11(21):3875-9. doi: 10.1021/bi00771a006.
2
Subunit structure of aspartate transcarbamylase from Escherichia coli.来自大肠杆菌的天冬氨酸转氨甲酰酶的亚基结构。
J Biol Chem. 1971 Mar 25;246(6):1644-57.
3
Kinetics of the interaction of methyl isonitrile with hemoglobin beta chains: measurement by nuclear magnetic resonance.
J Mol Biol. 1973 Dec 5;81(2):199-205. doi: 10.1016/0022-2836(73)90189-7.
4
Fluorotyrosine alkaline phosphatase from Escherichia coli: preparation, properties, and fluorine-19 nuclear magnetic resonance spectrum.
Proc Natl Acad Sci U S A. 1974 Feb;71(2):469-73. doi: 10.1073/pnas.71.2.469.
5
Fluorotyrosine alkaline phosphatase: internal mobility of individual tyrosines and the role of chemical shift anisotropy as a 19F nuclear spin relaxation mechanism in proteins.氟代酪氨酸碱性磷酸酶：单个酪氨酸的内部迁移率以及化学位移各向异性作为蛋白质中¹⁹F核自旋弛豫机制的作用。
J Mol Biol. 1975 Oct 15;98(1):121-53. doi: 10.1016/s0022-2836(75)80105-7.
6
Molecular dynamics and structure of the random coil and helical states of the collagen peptide, alpha 1-CB2, as determined by 13C magnetic resonance.通过13C磁共振测定的胶原蛋白肽α1-CB2的无规卷曲和螺旋状态的分子动力学与结构。
Biochemistry. 1975 Mar 11;14(5):887-900. doi: 10.1021/bi00676a004.
7
Concanavalin A: a stopped flow nuclear magnetic resonance study of conformational changes induced by Mn++, Ca++, and alpha-methyl-D-mannoside.
J Biol Chem. 1975 Mar 10;250(5):1618-24.
8
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9
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Biochemistry. 1975 Aug 26;14(17):3765-77. doi: 10.1021/bi00688a008.
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