Stiff M I, Olsen R G
Vet Immunol Immunopathol. 1984 Aug;7(1):1-9. doi: 10.1016/0165-2427(84)90022-9.
The feline one-way mixed leukocyte reaction (MLR) was accomplished by a microtechnique assay using gradient purified mononuclear leukocytes from the peripheral blood of specific-pathogen-free (SPF) cats. An eight day assay was required with an appropriate serum supplement of rabbit or cat serum and obtainable cell concentrations of 1 X 10(5) mononuclear cells per well of each responder and stimulator population. This assay was devised as an in vitro correlate of cell-mediated immunity and to demonstrate that feline histocompatibility differences can be detected in culture.
猫的单向混合淋巴细胞反应（MLR）通过一种微技术测定法来完成，该方法使用从无特定病原体（SPF）猫的外周血中梯度纯化的单核白细胞。该测定需要进行为期八天的实验，并添加适当的兔血清或猫血清作为补充，且每个反应细胞群和刺激细胞群每孔可获得的细胞浓度为1×10⁵个单核细胞。该测定法被设计为细胞介导免疫的体外相关指标，以证明在培养中可以检测到猫的组织相容性差异。
