Chang W M, Lin J K
Food Chem Toxicol. 1984 Nov;22(11):887-91. doi: 10.1016/0278-6915(84)90171-6.
The interconversion of zearalenone and zearalenol by rat erythrocytes in vitro has been investigated. The major metabolite obtained by incubating zearalenone with erythrocytes or whole blood from Sprague-Dawley rats was alpha-zearalenol. beta-Zearalenol was also formed but at levels several times lower than those of alpha-zearalenol. In the cell-free haemolysate NADPH was much more effective than NADH as a co-factor in the reduction of zearalenone. The maximal transformation of zearalenone to zearalenol by haemolysates occurred at pH 8.0. Both NAD+ and NADP+ were effective as co-factors in the oxidation of alpha-zearalenol to zearalenone. However, only NADP+ was effective as a co-factor in the oxidation of beta-zearalenol. Conversion of alpha-zearalenol and beta-zearalenol to the corresponding epimer was observed in both erythrocyte suspensions and in cell-free haemolysates. The significance of these findings to the metabolism of zearalenone in vivo is discussed.
已对大鼠红细胞在体外对玉米赤霉烯酮和玉米赤霉醇的相互转化进行了研究。将玉米赤霉烯酮与来自斯普拉格 - 道利大鼠的红细胞或全血一起孵育得到的主要代谢产物是α-玉米赤霉醇。也形成了β-玉米赤霉醇，但其水平比α-玉米赤霉醇低几倍。在无细胞溶血产物中，作为玉米赤霉烯酮还原反应的辅助因子，NADPH比NADH有效得多。溶血产物将玉米赤霉烯酮转化为玉米赤霉醇的最大转化率发生在pH 8.0时。NAD +和NADP +作为将α-玉米赤霉醇氧化为玉米赤霉烯酮的辅助因子均有效。然而，只有NADP +作为β-玉米赤霉醇氧化反应的辅助因子有效。在红细胞悬液和无细胞溶血产物中均观察到α-玉米赤霉醇和β-玉米赤霉醇向相应差向异构体的转化。讨论了这些发现对玉米赤霉烯酮体内代谢的意义。
