Richardson K E, Hagler W M, Hamilton P B
Appl Environ Microbiol. 1984 Jun;47(6):1206-9. doi: 10.1128/aem.47.6.1206-1209.1984.
Cultures of Fusarium roseium 'Gibbosum' on rice were treated with [14C]zearalenone, alpha[14C]zearalenol, or beta-[14C]zearalenol to determine whether a precursor-product relationship exists among these closely related fungal metabolites. Culture extracts were purified by silica gel column chromatography and fractionated by high-pressure liquid chromatography, and the level of radioactivity was determined. Within 7 days, the beta-[14C]zearalenol was converted to zearalenone, and no residual beta-[14C]zearalenol was detectable. Most of the alpha-[14C]zearalenol added was also converted into zearalenone with 14 days. In cultures treated with [14C]zearalenone, no radioactivity was noted in any other components.
用[14C]玉米赤霉烯酮、α-[14C]玉米赤霉烯醇或β-[14C]玉米赤霉烯醇处理水稻上的粉红镰孢菌“Gibbosum”培养物，以确定这些密切相关的真菌代谢产物之间是否存在前体-产物关系。培养物提取物通过硅胶柱色谱法纯化，并通过高压液相色谱法分级分离，然后测定放射性水平。在7天内，β-[14C]玉米赤霉烯醇转化为玉米赤霉烯酮，未检测到残留的β-[14C]玉米赤霉烯醇。添加的大部分α-[14C]玉米赤霉烯醇在14天内也转化为玉米赤霉烯酮。在用[14C]玉米赤霉烯酮处理的培养物中，未在任何其他成分中检测到放射性。
