Alcaraz G, Kinet J P, Kumar N, Wank S A, Metzger H
J Biol Chem. 1984 Dec 10;259(23):14922-7.
Above its critical micelle concentration, Triton X-114 in solution forms two phases at room temperature: a lower phase containing supramicellar aggregates and an upper phase largely depleted of detergent. This property of the detergent is potentially useful for separating under mild conditions proteins that bind detergent from those that do not (Bordier, C. (1981) J. Biol. Chem. 256, 1604-1607). We studied the distribution of the receptor for immunoglobulin E (IgE) and its subunits in the two phases. IgE and IgE complexed either with intact receptors or with the alpha chains of the receptor alone are principally partitioned into the upper phase, whereas the unliganded receptor as well as the isolated alpha, and especially the beta and gamma chains of the receptor, preferentially partition into the lower detergent phase. Chromatography of IgE and of the subunits of the receptor on a hydrophobic support showed that the beta and gamma chains have a considerably greater hydrophobic surface than the alpha chains or IgE. These results indicate that the distribution of a protein in the two phases of phase-separated Triton X-114 is not an all-or-none effect based upon whether it binds detergent or not. Rather, it reflects the overall balance between the hydrophobic and hydrophilic properties of the protein's surface.
在其临界胶束浓度以上，溶液中的曲拉通X-114在室温下形成两相：一相是含有超胶束聚集体的下层相，另一相是去污剂大量减少的上层相。这种去污剂的特性可能有助于在温和条件下将结合去污剂的蛋白质与不结合去污剂的蛋白质分离（博迪耶，C.（1981年）《生物化学杂志》256，1604 - 1607）。我们研究了免疫球蛋白E（IgE）受体及其亚基在这两相中的分布。与完整受体或仅与受体的α链复合的IgE主要分配到上层相，而未结合配体的受体以及分离出的α链，尤其是受体的β链和γ链，则优先分配到下层去污剂相。在疏水支持物上对IgE和受体亚基进行色谱分析表明，β链和γ链的疏水表面比α链或IgE大得多。这些结果表明，蛋白质在相分离的曲拉通X-114的两相中的分布不是基于其是否结合去污剂的全或无效应。相反，它反映了蛋白质表面疏水和亲水特性之间的总体平衡。
