Martin D E, Reece M, Reese A C
Cancer Invest. 1984;2(5):339-45. doi: 10.3109/07357908409040310.
Fibronectin (Fn) synergizes with macrophages (M phi) in inducing cytostasis and cytotoxicity of neoplastic cells in culture. Since heparin enhances Fn's opsonic activity in many systems, we investigated its effect on Fn-macrophage synergy in cytostasis. MCG-T14 (a spontaneous mouse mammary adenocarcinoma) cells (4 X 10(4] were added to wells both with and without C. parvum activated M phi monolayers. To these cultures were added increasing concentrations of Fn with or without heparin. Fn synergizes with both M phi S and heparin in inhibiting tumor cell growth. The combined cytostatic effect of Fn, heparin and M phi S is more than additive. In other experiments, MCG-T14 cells were pre-incubated for 2 hr with Fn, washed free of Fn, and treated as above. The results of these experiments were similar to coculture experiments, but the effect of heparin was even more pronounced. Dermatan sulfate and hyaluronic acid had a variable effect on Fn and Fn-macrophage induced cytostasis.
纤连蛋白（Fn）与巨噬细胞（M phi）协同作用，在培养中诱导肿瘤细胞的细胞生长停滞和细胞毒性。由于肝素在许多系统中增强了Fn的调理活性，我们研究了其对Fn-巨噬细胞在细胞生长停滞方面协同作用的影响。将MCG-T14（一种自发性小鼠乳腺腺癌）细胞（4×10⁴）添加到有和没有经微小隐孢子虫激活的M phi单层的孔中。向这些培养物中添加有或没有肝素的递增浓度的Fn。Fn与M phi S和肝素协同抑制肿瘤细胞生长。Fn、肝素和M phi S的联合细胞生长停滞作用大于相加作用。在其他实验中，MCG-T14细胞先用Fn预孵育2小时，洗去Fn，然后按上述方法处理。这些实验的结果与共培养实验相似，但肝素的作用更为明显。硫酸皮肤素和透明质酸对Fn和Fn-巨噬细胞诱导的细胞生长停滞有可变的影响。
