对培养的新生大鼠心脏细胞中肾上腺素积累和肾上腺素能反应性的初步研究。
Initial investigations into adrenaline accumulation and adrenergic responsiveness in cultured neonatal rat heart cells.
作者信息
Walker M J
出版信息
Br J Pharmacol. 1978 Feb;62(2):185-93. doi: 10.1111/j.1476-5381.1978.tb08444.x.
Abstract
1 Accumulation of tritiated adrenaline (and/or its products) has been studied by a variety of techniques in viable, attached and free-floating myoblasts cultured from neonatal rat heart cells. 2 Accumulation increased with the number of cells used, the culture age of cells, incubation time and the concentration of adrenaline in the incubation mixture. 3 Accumulation-concentration data were not linearly correlated with dose-response data. 4 Accumulation did not correlate well with beta-adrenoceptor responsiveness in a number of situations of varying responsiveness. 5 Accumulation was not blocked by a variety of uptake blocking agents or alpha- and beta-adrenoceptor antagonists. 6 A much smaller accumulation process was observed in fibroblasts which respond to adrenaline with reversible morphological changes.
摘要
- 已采用多种技术研究了新生大鼠心脏细胞培养的存活、贴壁和游离成肌细胞中氚化肾上腺素（和/或其产物）的蓄积情况。2. 蓄积量随所用细胞数量、细胞培养时间、孵育时间以及孵育混合物中肾上腺素浓度的增加而增加。3. 蓄积-浓度数据与剂量-反应数据并非线性相关。4. 在多种反应性不同的情况下，蓄积与β-肾上腺素能受体反应性的相关性不佳。5. 多种摄取阻断剂以及α和β肾上腺素能受体拮抗剂均未阻断蓄积。6. 在对肾上腺素产生可逆形态学变化反应的成纤维细胞中观察到的蓄积过程要小得多。
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