Acosta D, Sorensen E M, Anuforo D C, Mitchell D B, Ramos K, Santone K S, Smith M A
In Vitro Cell Dev Biol. 1985 Sep;21(9):495-504. doi: 10.1007/BF02620841.
A major goal of our laboratory has been the development of primary culture systems that retain differentiated functions and responses characteristic of intact tissues in vivo. Specifically, we have developed cellular models of primary cultures of rat heart, liver, and kidney cells to explore the mechanisms by which drugs or chemicals may be toxic to key organs of the body and to develop new techniques by which xenobiotics may be evaluated or identified as potential toxicants to living systems. The purpose of this paper is to describe our rationale and approach to the study of target organ toxicology with in vitro cellular systems.
我们实验室的一个主要目标是开发原代培养系统，该系统能够保留体内完整组织的分化功能和特征性反应。具体而言，我们已经建立了大鼠心脏、肝脏和肾脏细胞原代培养的细胞模型，以探究药物或化学物质可能对身体关键器官产生毒性的机制，并开发新的技术，通过这些技术可以评估或鉴定外源性物质是否为对生物系统的潜在毒物。本文的目的是描述我们利用体外细胞系统研究靶器官毒理学的基本原理和方法。
