Atkinson H A, Miller K
Toxicol Lett. 1984 Nov;23(2):215-21. doi: 10.1016/0378-4274(84)90129-2.
Deoxynivalenol (DON), 3-acetyldeoxynivalenol (acetylDON) and zearalenone (Ze) were examined for their in vitro effect on mitogen-induced lymphocyte blastogenesis using rat or human peripheral blood lymphocytes (PBL). A dose-dependent reduction of lymphocyte proliferation was demonstrated for each mycotoxin. However, the inhibitory effect of DON was significantly higher than that of the acetylated compound. Concentrations of 90 ng/ml and 220 ng/ml inhibited rat and human lymphocyte blastogenesis by 50%, respectively, whereas 450 ng/ml and 1060 ng/ml acetylDON were required to produce the same effect. The amount of Ze necessary to inhibit blastogenesis by 50% was 250 times greater than required for DON. There was no evidence of cell death and combinations of DON and Ze did not alter the expected response.
使用大鼠或人外周血淋巴细胞（PBL），检测了脱氧雪腐镰刀菌烯醇（DON）、3-乙酰脱氧雪腐镰刀菌烯醇（乙酰DON）和玉米赤霉烯酮（Ze）对丝裂原诱导的淋巴细胞增殖的体外作用。每种霉菌毒素均显示出淋巴细胞增殖的剂量依赖性降低。然而，DON的抑制作用明显高于乙酰化化合物。90 ng/ml和220 ng/ml的浓度分别抑制大鼠和人淋巴细胞增殖50%，而需要450 ng/ml和1060 ng/ml的乙酰DON才能产生相同效果。抑制增殖50%所需的Ze量比DON所需量高250倍。没有细胞死亡的证据，并且DON和Ze的组合未改变预期反应。
