Visconti A, Minervini F, Lucivero G, Gambatesa V
Istituto Tossine e Micotossine da Parassiti Vegetali, CNR, Bari, Italy.
Mycopathologia. 1991 Mar;113(3):181-6. doi: 10.1007/BF00436128.
A colorimetric MTT (tetrazolium salt) cleavage test was used to evaluate cytotoxicity of twenty-three Fusarium mycotoxins on two cultured human cell lines (K-562 and MIN-GL1) as well as their inhibitory effect on proliferation of phytohemagglutinin-stimulated human peripheral blood lymphocytes. The values of 50% inhibition of lymphocyte blastogenesis were very close to the 50% cytotoxic doses observed with the more sensitive cell line (MIN-GL1). T-2 toxin was the most cytotoxic with CD50 and ID50 values less than 1 ng/ml. Type A trichothecenes were the most cytotoxic followed by the type B trichothecenes; the non-trichothecenes were the least cytotoxic. The MTT cleavage test, in conjunction with cell culture, is a simple and rapid bioassay to evaluate cytotoxicity and immunotoxicity of Fusarium mycotoxins.
采用比色法MTT（四唑盐）裂解试验评估了23种镰刀菌霉菌毒素对两种培养的人细胞系（K-562和MIN-GL1）的细胞毒性，以及它们对植物血凝素刺激的人外周血淋巴细胞增殖的抑制作用。淋巴细胞增殖抑制率50%时的值与在更敏感的细胞系（MIN-GL1）中观察到的50%细胞毒性剂量非常接近。T-2毒素的细胞毒性最强，其半数细胞毒性剂量（CD50）和半数抑制剂量（ID50）值小于1 ng/ml。A型单端孢霉烯族毒素的细胞毒性最强，其次是B型单端孢霉烯族毒素；非单端孢霉烯族毒素的细胞毒性最弱。MTT裂解试验结合细胞培养，是一种评估镰刀菌霉菌毒素细胞毒性和免疫毒性的简单快速生物测定法。
