Inman R B
Biochim Biophys Acta. 1984 Dec 14;783(3):205-15. doi: 10.1016/0167-4781(84)90030-7.
Methodology is described that will permit the study of the effect of various drugs on development and DNA replication in the cleavage nuclei of Drosophila eggs. It is shown that permeabilized eggs can be exposed to an aqueous incubation medium for up to 30 min without measureable effects on development and that such incubations can be performed with eggs that have a relatively sharp age distribution. The effect on development and viability of a variety of drugs has been examined as an aid for future studies directed toward achieving synchronous development in a population of eggs and electron microscopic studies of DNA replication in the presence of various drugs.
本文描述了一种方法，该方法可用于研究各种药物对果蝇卵裂核发育和DNA复制的影响。研究表明，通透化的卵可以在水性孵育培养基中暴露长达30分钟，而对发育没有可测量的影响，并且这种孵育可以在年龄分布相对集中的卵上进行。已经研究了多种药物对发育和活力的影响，以辅助未来旨在使一群卵实现同步发育的研究，以及在各种药物存在的情况下对DNA复制进行电子显微镜研究。
