Engstrom F L, Kemp J W, Woodbury D M
Epilepsia. 1984 Dec;25(6):759-64. doi: 10.1111/j.1528-1157.1984.tb03488.x.
DBA/2J mice are susceptible to audiogenic seizures (ASs) in an age-dependent manner, susceptibility being maximal at 21 days of age and declining thereafter. DBA, as compared with AS-resistant C57BL/6J (C57) mice, had higher carbonic anhydrase (CA) activity in cerebral cortex, brainstem, and cerebellum homogenates at 21 days of age. CA activity was also increased in cytosolic (82%), microsomal (167%), and myelin (68%) subcellular fractions from cerebral cortex, and in cytosolic (51%) and mitochondrial (102%) fractions from brainstem of DBA mice at 21 days of age. In addition, DBA mice had a higher Na+, K+-ATPase activity in myelin from cerebral cortex, and a lower HCO3--ATPase activity in mitochondria from brainstem. The differences in CA activity in the cerebral cortex and in HCO3--ATPase were not present at 110 days of age, when DBA mice are no longer susceptible to ASs. Because CA and HCO3--ATPase are involved in maintaining a proper ionic environment for neuronal function, these data suggest that alterations in activity of these enzymes are related to the age-dependent changes in AS susceptibility in DBA mice.
DBA/2J小鼠对听源性癫痫（AS）的易感性呈年龄依赖性，在21日龄时易感性最高，此后逐渐下降。与抗AS的C57BL/6J（C57）小鼠相比，DBA小鼠在21日龄时大脑皮质、脑干和小脑匀浆中的碳酸酐酶（CA）活性更高。在21日龄的DBA小鼠中，大脑皮质的胞质（82%）、微粒体（167%）和髓磷脂（68%）亚细胞组分以及脑干的胞质（51%）和线粒体（102%）组分中的CA活性也有所增加。此外，DBA小鼠大脑皮质髓磷脂中的Na+，K+-ATP酶活性较高，脑干线粒体中的HCO3--ATP酶活性较低。在110日龄时，DBA小鼠不再易患AS，此时大脑皮质中CA活性和HCO3--ATP酶活性的差异不复存在。由于CA和HCO3--ATP酶参与维持神经元功能的适当离子环境，这些数据表明这些酶活性的改变与DBA小鼠AS易感性的年龄依赖性变化有关。
