Howles A, Cox K O
Aust J Exp Biol Med Sci. 1984 Aug;62 ( Pt 4):429-43. doi: 10.1038/icb.1984.41.
Erythrocyte autoantibodies and autoantibody-specific suppressor cells are elicited in mice injected with rat erythrocytes. Here it is shown, first, that modified rat erythrocytes elicit suppressor cells independently of autoantibody production. Haemoglobin-free membranes of rat erythrocytes, but not soluble lysates of rat erythrocytes, elicited autoantibodies and suppressor cells. Solubilization of these rat erythrocyte membranes with solutions of Triton-X-114 produced membrane fractions which induced autoantibody-specific suppression in the absence of detectable autoantibody production. These results are compatible with the view that the activation of suppressor cells in this model involves recognition of structures on the rat erythrocytes. Secondly, trinitrophenyl (TNP) was attached to rat erythrocytes in various doses in attempts to make the immunogenicity of TNP similar to that of the cross-reactive determinants on rat and mouse erythrocytes, to determine whether TNP-specific suppressor cells were produced. The results show that at various doses of TNP, including those at which antibody production to TNP could not be detected, rat erythrocytes coated with TNP induced autoantibody-specific suppressor cells but not suppressor cells with specificity for antibodies to TNP.
给小鼠注射大鼠红细胞后，可诱导出红细胞自身抗体和自身抗体特异性抑制细胞。本文首先表明，修饰后的大鼠红细胞可独立于自身抗体产生而诱导抑制细胞。大鼠红细胞的无血红蛋白膜可诱导自身抗体和抑制细胞，而大鼠红细胞的可溶性裂解物则不能。用Triton-X-114溶液溶解这些大鼠红细胞膜产生的膜组分，在未检测到自身抗体产生的情况下诱导了自身抗体特异性抑制。这些结果与该模型中抑制细胞的激活涉及对大鼠红细胞上结构的识别这一观点相符。其次，将三硝基苯基（TNP）以不同剂量连接到大鼠红细胞上，试图使TNP的免疫原性与大鼠和小鼠红细胞上的交叉反应性决定簇相似，以确定是否产生了TNP特异性抑制细胞。结果表明，在不同剂量的TNP下，包括那些检测不到针对TNP的抗体产生的剂量，包被有TNP的大鼠红细胞诱导了自身抗体特异性抑制细胞，但未诱导对TNP抗体具有特异性的抑制细胞。
