Calderón J, de Lourdes Muñoz M, Acosta H M
J Exp Med. 1980 Jan 1;151(1):184-93. doi: 10.1084/jem.151.1.184.
Polyspecific antibodies bound to Entamoeba induced surface redistribution of membrane components toward the uroid region. Capping of surface antigens was obtained with a single layer of antibodies in E. histolytica and E. invadens. This surface segregation progressed to a large accumulation of folded plasma membrane that extruded as a defined vesicular cap. A spontaneous release of the cap at the end of the capping process took place. These released caps contained most of the antibodies that originally bound to the whole cell surface. Two-thirds of radiolabeled antibodies bound to the surface of E. histolytica were released into the medium in 2 h. Successive capping induced by repeated exposure of E. invadens to antibodies produced conglomerates of folded surface membrane, visualized as stacked caps, in proportion to the number of antibody exposures. These results indicate the remarkable ability of Entamoeba to rapidly regenerate substantial amounts of plasma membbrane. The properties of surface redistribution, liberation of caps, and plasma membrane regeneration, may contribute to the survival of the parasite in the host during infection.
与溶组织内阿米巴结合的多特异性抗体诱导膜成分向尾状区进行表面重新分布。在溶组织内阿米巴和侵袭内阿米巴中，单层抗体可实现表面抗原的封帽现象。这种表面分离发展为大量折叠质膜的聚集，形成明确的囊泡状帽并挤出。在封帽过程结束时，帽会自发释放。这些释放的帽包含了最初结合在整个细胞表面的大部分抗体。两小时内，与溶组织内阿米巴表面结合的三分之二放射性标记抗体被释放到培养基中。侵袭内阿米巴反复暴露于抗体诱导的连续封帽产生了折叠表面膜的聚集体，表现为堆叠的帽，其数量与抗体暴露次数成正比。这些结果表明溶组织内阿米巴具有快速再生大量质膜的显著能力。表面重新分布、帽的释放和质膜再生的特性可能有助于寄生虫在感染宿主期间的存活。
