Espinosa-Cantellano M, Martínez-Palomo A
Department of Experimental Pathology, Centro de Investigación y de Estudios Avanzados del IPN, Mexico, D.F., Mexico.
Exp Parasitol. 1994 Nov;79(3):424-35. doi: 10.1006/expr.1994.1104.
Binding of antibodies to the surface of the parasitic protozoan Entamoeba histolytica induces capping and release of antigen-antibody complexes, in a process that may be an effective means of evading the host immune response. To analyze the mechanism of capping in E. histolytica trophozoites, optimal conditions were determined for cap induction by sera from patients with amebic liver abscess, by polyclonal anti-amebic antibodies, and by concanavalin A. Cocapping of actin and myosin was documented by immunofluorescent staining of capped amebas using phalloidin, monoclonal anti-actin antibodies, and polyclonal anti-myosin antibodies. Immunoblots confirmed the presence of actin and myosin in isolated caps. These observations demonstrate that amebic cytoskeletal components participate in E. histolytica capping of surface receptors.
抗体与寄生原生动物溶组织内阿米巴表面的结合会诱导抗原-抗体复合物的帽化和释放，这一过程可能是逃避宿主免疫反应的有效手段。为了分析溶组织内阿米巴滋养体中帽化的机制，确定了由阿米巴肝脓肿患者血清、多克隆抗阿米巴抗体和伴刀豆球蛋白A诱导帽化的最佳条件。使用鬼笔环肽、单克隆抗肌动蛋白抗体和多克隆抗肌球蛋白抗体对帽化的阿米巴进行免疫荧光染色，记录了肌动蛋白和肌球蛋白的共帽化。免疫印迹证实了分离出的帽中存在肌动蛋白和肌球蛋白。这些观察结果表明，阿米巴细胞骨架成分参与了溶组织内阿米巴表面受体的帽化。
