Sultatos L G, Vesell E S
Proc Natl Acad Sci U S A. 1980 Jan;77(1):600-3. doi: 10.1073/pnas.77.1.600.
Cytochrome P-450 content (nmol/g of liver) differed within regions of rat liver according to proximity to intrahepatically implanted Morris hepatoma 7795 or 5123D. Liver adjacent to tumor had higher microsomal cytochrome P-450 content, decreased DNA content (mg/g of liver), and unaltered cytochrome c reductase activity compared to histologically indistinguishable liver far-removed from the tumor. Liver either adjacent to or far-removed from tumor contained markedly more cytochrome P-450 and higher cytochrome c reductase activity but less DNA than transplanted Morris hepatomas 7795 and 5123D that were grown intrahepatically. Compared to intramuscular implants of these same tumors, intrahepatically implanted Morris hepatomas 7795 and 5123D had increased cytochrome P-450 content. Tumor-containing liver from two human subjects revealed regional changes in cytochrome P-450-mediated monooxygenases similar to those observed in rats. These results suggest that histomorphically nontumorous mammalian liver directly adjacent to intrahepatic tumors exhibits previously unsuspected biochemical alterations.
根据与肝内植入的莫里斯肝癌7795或5123D的接近程度，大鼠肝脏各区域的细胞色素P - 450含量（nmol/g肝脏）有所不同。与组织学上无法区分的远离肿瘤的肝脏相比，靠近肿瘤的肝脏微粒体细胞色素P - 450含量更高，DNA含量（mg/g肝脏）降低，细胞色素c还原酶活性未改变。与肝内生长的移植性莫里斯肝癌7795和5123D相比，无论是靠近肿瘤还是远离肿瘤的肝脏，其细胞色素P - 450含量明显更高，细胞色素c还原酶活性更高，但DNA含量更低。与这些相同肿瘤的肌肉内植入相比，肝内植入的莫里斯肝癌7795和5123D的细胞色素P - 450含量增加。来自两名人类受试者的含肿瘤肝脏显示出细胞色素P - 450介导的单加氧酶的区域变化，类似于在大鼠中观察到的变化。这些结果表明，与肝内肿瘤直接相邻的组织形态学上无肿瘤的哺乳动物肝脏表现出以前未被怀疑的生化改变。
