Joho R, Weissman I L, Early P, Cole J, Hood L
Proc Natl Acad Sci U S A. 1980 Feb;77(2):1106-10. doi: 10.1073/pnas.77.2.1106.
We studied the organization of the kappa light chain genes in germ-line (sperm) and somatic (embryo) tissues. We constructed a plasmid containing a DNA insert coding for the kappa chain MOPC 167 and used the Southern blotting technique to determine the organization of kappa variable and constant region genes. In the haploid genome of the mouse there is only one constant region gene detectable and it has the same organization in sperm and embryo DNAs. There are several variable region genes in sperm and embryo that are related to the Vk167 gene. The organization of the V genes in sperm and embryo DNAs is identical. These results show that there is no rearrangement of variable region genes (or "minigenes") during early embryogenesis.
我们研究了种系（精子）和体细胞（胚胎）组织中κ轻链基因的组织形式。我们构建了一个含有编码κ链MOPC 167的DNA插入片段的质粒，并使用Southern印迹技术来确定κ可变区和恒定区基因的组织形式。在小鼠的单倍体基因组中，只能检测到一个恒定区基因，并且它在精子和胚胎DNA中的组织形式相同。精子和胚胎中有几个与Vk167基因相关的可变区基因。精子和胚胎DNA中V基因的组织形式是相同的。这些结果表明，在早期胚胎发生过程中可变区基因（或“小基因”）没有重排。
Zotero 插件
