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种系和体细胞组织中κ轻链基因的组织方式。

Organization of kappa light chain genes in germ-line and somatic tissue.

作者信息

Joho R, Weissman I L, Early P, Cole J, Hood L

出版信息

Proc Natl Acad Sci U S A. 1980 Feb;77(2):1106-10. doi: 10.1073/pnas.77.2.1106.

DOI:10.1073/pnas.77.2.1106
PMID:6244580
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC348433/
Abstract

We studied the organization of the kappa light chain genes in germ-line (sperm) and somatic (embryo) tissues. We constructed a plasmid containing a DNA insert coding for the kappa chain MOPC 167 and used the Southern blotting technique to determine the organization of kappa variable and constant region genes. In the haploid genome of the mouse there is only one constant region gene detectable and it has the same organization in sperm and embryo DNAs. There are several variable region genes in sperm and embryo that are related to the Vk167 gene. The organization of the V genes in sperm and embryo DNAs is identical. These results show that there is no rearrangement of variable region genes (or "minigenes") during early embryogenesis.

摘要

我们研究了种系（精子）和体细胞（胚胎）组织中κ轻链基因的组织形式。我们构建了一个含有编码κ链MOPC 167的DNA插入片段的质粒，并使用Southern印迹技术来确定κ可变区和恒定区基因的组织形式。在小鼠的单倍体基因组中，只能检测到一个恒定区基因，并且它在精子和胚胎DNA中的组织形式相同。精子和胚胎中有几个与Vk167基因相关的可变区基因。精子和胚胎DNA中V基因的组织形式是相同的。这些结果表明，在早期胚胎发生过程中可变区基因（或“小基因”）没有重排。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c361/348433/c9193ef1969d/pnas00665-0424-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c361/348433/da45cbdd7b33/pnas00665-0423-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c361/348433/0284e6e96393/pnas00665-0423-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c361/348433/342442e9d86f/pnas00665-0423-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c361/348433/c9193ef1969d/pnas00665-0424-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c361/348433/da45cbdd7b33/pnas00665-0423-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c361/348433/0284e6e96393/pnas00665-0423-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c361/348433/342442e9d86f/pnas00665-0423-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c361/348433/c9193ef1969d/pnas00665-0424-a.jpg

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Organization of kappa light chain genes in germ-line and somatic tissue.种系和体细胞组织中κ轻链基因的组织方式。
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引用本文的文献

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A Proposed New Nomenclature for the Immunoglobulin Genes of .一种新的免疫球蛋白基因命名法的建议。
Front Immunol. 2019 Dec 18;10:2961. doi: 10.3389/fimmu.2019.02961. eCollection 2019.
2
Characterization of a V kappa family in Mus musculus castaneus: sequence analysis.小家鼠栗色亚种中一个Vκ家族的特征分析：序列分析
Immunogenetics. 1993;37(6):426-36. doi: 10.1007/BF00222466.
3
Characterization of a V kappa family in Mus musculus castaneus: expansion at the subset level.小家鼠栗色亚种中一个Vκ基因家族的特征：在亚群水平上的扩增

本文引用的文献

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Somatic diversification is required to generate the V kappa genes of MOPC 511 and MOPC 167 myeloma proteins.体细胞多样化是产生MOPC 511和MOPC 167骨髓瘤蛋白的Vκ基因所必需的。
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7674-8. doi: 10.1073/pnas.78.12.7674.
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Mapping of immunoglobulin variable region genes: relationship to the 'deletion' model of immunoglobulin gene rearrangement.免疫球蛋白可变区基因的图谱绘制：与免疫球蛋白基因重排的“缺失”模型的关系
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