Vκ种系基因多基因家族的进化
Evolution of a multigene family of V kappa germ line genes.
作者信息
Joho R, Gershenfeld H, Weissman I L
出版信息
EMBO J. 1984 Jan;3(1):185-91. doi: 10.1002/j.1460-2075.1984.tb01782.x.
Abstract
We have isolated a series of related V kappa germ line genes from a BALB/c sperm DNA library. DNA sequence analysis of four members of this V kappa 24 multigene family implies that three V kappa genes are functional whereas the fourth one (psi V kappa 24) is a pseudogene. The prototype gene (V kappa 24) encodes the variable region gene segment expressed in an immune response against phosphorylcholine. The other two functional genes (V kappa 24A and V kappa 24B) may be expressed against streptococcal group A carbohydrate. The time of divergence of the four genes was estimated by the rate of synonymous nucleotide changes. This implies that an ancestral gene has duplicated approximately 33-35 million years ago and a subsequent gene duplication event has occurred approximately 23 million years ago.
摘要
我们从BALB/c小鼠精子DNA文库中分离出了一系列相关的Vκ种系基因。对这个Vκ24多基因家族的四个成员进行DNA序列分析表明，其中三个Vκ基因是有功能的，而第四个基因（ψVκ24）是一个假基因。原型基因（Vκ24）编码在针对磷酸胆碱的免疫反应中表达的可变区基因片段。另外两个有功能的基因（Vκ24A和Vκ24B）可能在针对A组链球菌碳水化合物的免疫反应中表达。通过同义核苷酸变化率估计了这四个基因的分歧时间。这意味着一个祖先基因大约在3300万至3500万年前发生了复制，随后在大约2300万年前发生了一次基因复制事件。
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本文引用的文献
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Two kappa immunoglobulin genes are expressed in the myeloma S107.两个κ免疫球蛋白基因在骨髓瘤S107中表达。
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A single VH gene segment encodes the immune response to phosphorylcholine: somatic mutation is correlated with the class of the antibody.单个VH基因片段编码对磷酸胆碱的免疫反应：体细胞突变与抗体类别相关。
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