Robinson L H, Landy A
Gene. 1977 Sep;2(1):1-31. doi: 10.1016/0378-1119(77)90019-1.
The site-specific restriction endonucleases isolated from Hemophilus influenzae strains Rc (HincII) and Rd (HindII + III), and Hemophilus parainfluenzae (HpaI) were used to digest bacteriophage lambda DNA into 34, 40, and 15 specific fragments, respectively. The sites cleaved by each of these enzymes were localized on the lambda physical map and the fragments resulting from these cleavages were electrophoretically identified on gels by (1) analysis of the digestion profiles of deletion and transducing derivatives of lambda; and (2) digesting individual fragments produced by one restriction endonuclease with another restriction endonuclease. This paper presents the HindII, HindIII, and HpaI restriction fragment maps for the entire lambda genome, and the data used to derive these maps for the region of the lambda genome between the attachment site (at 57.3% lambda) and the right vegetative end (100% lambda). The data for mapping the left arm of lambda may be found in the accompanying paper (Robinson and Landy, 1977).
从流感嗜血杆菌Rc株（HincII）和Rd株（HindII + III）以及副流感嗜血杆菌（HpaI）中分离出的位点特异性限制性内切酶，分别用于将噬菌体λ DNA消化成34、40和15个特异性片段。这些酶各自切割的位点定位在λ物理图谱上，并且通过以下方法在凝胶上电泳鉴定这些切割产生的片段：（1）分析λ缺失和转导衍生物的消化图谱；（2）用另一种限制性内切酶消化由一种限制性内切酶产生的单个片段。本文给出了整个λ基因组的HindII、HindIII和HpaI限制性片段图谱，以及用于推导λ基因组在附着位点（位于λ的57.3%处）和右侧营养末端（100%λ）之间区域的这些图谱的数据。关于λ左臂图谱绘制的数据可见随附论文（罗宾逊和兰迪，1977年）。
