Lange-Gustafson B, Rhoades M
J Virol. 1979 Jun;30(3):923-8. doi: 10.1128/JVI.30.3.923-928.1979.
Cleavage maps of bacteriophage BF23 DNA have been constructed for the restriction endonucleases SalI (3 fragments), BamHI (5 fragments), EcoRI, (8 fragments), BalI (13 fragments), and HpaI (49 fragments, 32 of which have been ordered). The maps were determined by (i) analysis of deletion mutants, (ii) digestion with two endonucleases, (iii) digestion of isolated fragments with a second enzyme, (iv) analysis of partial digests, and (v) digestion after treatment with lambda exonuclease.
已构建了噬菌体BF23 DNA的切割图谱,所用的限制性内切酶有SalI(3个片段)、BamHI(5个片段)、EcoRI(8个片段)、BalI(13个片段)和HpaI(49个片段,其中32个已排序)。这些图谱是通过以下方法确定的:(i) 缺失突变体分析;(ii) 用两种内切酶消化;(iii) 用第二种酶消化分离的片段;(iv) 部分消化分析;以及(v) 用λ外切核酸酶处理后进行消化。